Journal: Nutrients

Article Title: Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV- neu T) in Mice with Transplanted Salivary Gland Carcinoma Cells

doi: 10.3390/nu12051417

Figure Lengend Snippet: Interferon (IFN)-γ and Interleukin (IL)-2 release from splenocytes treated with Neu peptides. The release of cytokines was assessed in splenocytes collected at sacrifice from treated mice after 96 h stimulation in vitro with Neu peptides. The results show the cytokine release induced by an irrelevant peptide (gag) subtracted from the IFN-γ or IL-2 release of each sample, in two experiments performed in duplicate (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001; one-way-ANOVA, Tukey’s multiple comparison). ( A , C ) show the release of IFN-γ or IL-2 when all peptides were used in the assay, while ( B , D ) show the contribution of each peptide to the T-cell cytokine release. Results represent three independent experiments of T-cell stimulation with Neu peptides.

Article Snippet: Cytokine release, IHC data, and distribution of cell survival were preliminarily verified using the Kolmogorov–Smirnov test and data sets were analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s test with GraphPad Prism 5.00.288.

Techniques: In Vitro, Comparison, Cell Stimulation

Journal: Nutrients

Article Title: Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV- neu T) in Mice with Transplanted Salivary Gland Carcinoma Cells

doi: 10.3390/nu12051417

Figure Lengend Snippet: Helper T cells infiltrating tumors in BALB- neu T mice after treatments. Tumor tissues from three mice in each group were analyzed with IHC for CD4 expression. ( A ) Positive cell count/field averaging 10 representative microscopic fields (mean ± SD, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; one-way-ANOVA, Tukey’s multiple comparison). ( B ) Representative digital images (20×), scale bar represents 100 µm. T H : helper T lymphocytes, CUR: curcumin.

Article Snippet: Cytokine release, IHC data, and distribution of cell survival were preliminarily verified using the Kolmogorov–Smirnov test and data sets were analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s test with GraphPad Prism 5.00.288.

Techniques: Expressing, Cell Counting, Comparison

Journal: Nutrients

Article Title: Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV- neu T) in Mice with Transplanted Salivary Gland Carcinoma Cells

doi: 10.3390/nu12051417

Figure Lengend Snippet: Cytotoxic T cells infiltrating tumors in BALB- neu T mice after treatments. Tumor tissues from three mice in each group were analyzed with IHC for CD8 expression. ( A ) Positive cell count/field averaging 10 representative microscopic fields (mean ± SD, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; one-way-ANOVA, Tukey’s multiple comparison). ( B ) Representative digital images (20×), scale bar represents 100 µm. T C : cytotoxic T lymphocytes, CUR: curcumin.

Article Snippet: Cytokine release, IHC data, and distribution of cell survival were preliminarily verified using the Kolmogorov–Smirnov test and data sets were analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s test with GraphPad Prism 5.00.288.

Techniques: Expressing, Cell Counting, Comparison

Journal: Nutrients

Article Title: Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV- neu T) in Mice with Transplanted Salivary Gland Carcinoma Cells

doi: 10.3390/nu12051417

Figure Lengend Snippet: Apoptotic cells within the tumors of BALB- neu T-treated mice. Tumor tissues from three mice in each group were analyzed by IHC analysis for cleaved caspase-3 expression (arrow). ( A ) Positive cell count/field averaging 10 representative microscopic fields (mean ± SD, *** p ≤ 0.001; one-way-ANOVA, Tukey’s multiple comparison). ( B ) Representative digital images (40×), scale bar represents 50 µm. CUR: curcumin.

Article Snippet: Cytokine release, IHC data, and distribution of cell survival were preliminarily verified using the Kolmogorov–Smirnov test and data sets were analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s test with GraphPad Prism 5.00.288.

Techniques: Expressing, Cell Counting, Comparison

Journal: Nutrients

Article Title: Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV- neu T) in Mice with Transplanted Salivary Gland Carcinoma Cells

doi: 10.3390/nu12051417

Figure Lengend Snippet: In vitro effect of mAb 4D5 on Head and neck cancer (HNC) cells. ( A ) FACS analysis was performed on HNC cell lines of the tongue (SCC-15), pharynx (FaDu), or salivary gland (A-253) using mAb 4D5 (5 μg/mL). MOPC-21 (5 μg/mL) was used as a control. ( B ) Cell growth was assessed by a sulforhodamine B (SRB) assay after 48 and 72 h of treatment with mAb 4D5 at different concentrations (1.25, 2.5, 5 μg/mL). MOPC-21 (5 μg/mL) was used as a control (CTRL). The results are expressed as the mean ± SD of three independent experiments performed in triplicate (* p ≤ 0.05, *** p ≤ 0.001, compared with the control cultures; one-way-ANOVA, Tukey’s multiple comparison).

Article Snippet: Cytokine release, IHC data, and distribution of cell survival were preliminarily verified using the Kolmogorov–Smirnov test and data sets were analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s test with GraphPad Prism 5.00.288.

Techniques: In Vitro, Control, Sulforhodamine B Assay, Comparison

Journal: Plants

Article Title: An HLD Model for Tomato Bacterial Canker Focusing on Epidemics of the Pathogen Due to Cutting by Infected Scissors

doi: 10.3390/plants11172253

Figure Lengend Snippet: Relationship between populations of Cmm and probability of tomato bacterial canker (TBC) infection. ( A ) Populations of Cmm in tomato bacterial canker-infected plants, grouped according to symptoms (symptomless, early wilt, and late wilt). The center bar of the boxplot is the median, the lower and upper horizontal bars are the 25th and 75th percentile, and whiskers show the 95% range. The values of log 10 CFU/g plant tissues with three different symptoms were not significantly different (ns, p > 0.05, ANOVA). ( B ) Proportion of diseased plants inoculated with different concentrations of Cmm . Error bars show standard error of mean (SEM). Different letters indicate a significant difference from the other bars in different concentrations of Cmm ( p ≤ 0.05, Tukey’s HSD test).

Article Snippet: ANOVA and Tukey’s multiple test ( n = 4) were performed using RStudio.

Techniques: Infection

Journal: Bio-protocol

Article Title: Analyses of Root-secreted Acid Phosphatase Activity in Arabidopsis

doi: 10.21769/BioProtoc.2202

Figure Lengend Snippet: Root-associated APase activities (A) and APase activities in the medium (B) were determined with pNPP as the substrate. Values are means with SE. A one-way ANOVA analysis was carried out for the whole data set, and post hoc comparisons were conducted using the SPSS Tukey HSD test at P < 0.05. Within each of the four plots, values with different letters are significantly different. Adapted from Wang et al. (2014) .

Article Snippet: A one-way ANOVA analysis was carried out for the whole data set, and post hoc comparisons were conducted using the SPSS Tukey HSD test at P < 0.05.

Techniques: